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A framework is proposed using multi-stream phycocyanin probe readings to manage cyanobacterial risks at the source water and across the drinking water treatment processes. , In situ phycocyanin (PC) probes have been deployed as a cost-effective and efficient way to monitor cyanobacterial (CB) abundance in drinking water sources and to identify periods of potential risk at drinking water treatment plants (DWTPs). Monitoring CB removal efficacies in near real-time by sequentially using a single probe for multiple streams across the treatment plant provides a more useful assessment of CB risk breakthrough in treated water. Removal efficacies were measured in three DWTPs using integrated mass fluxes estimated from PC readings and grab sample total CB biovolume estimations in raw, clarified, filtered, and treated water. Selective CB species removal during the treatment processes was also evaluated. In addition, relationships between physio-chemical parameters (turbidity, pH, dissolved oxygen, conductivity, chlorophyll-a and temperature) and PC across the treatment processes were investigated. Finally, a framework to use in situ multi-stream PC monitoring, gathering data across the treatment chain, is proposed to manage risks of CB cells breakthrough in treated water. Estimates of 2 hour moving average PC metrics are proposed to provide short term alert in raw water and establish periods of treatment vulnerability or dysfunction, while daily mean PC values can be used to estimate total and process specific log removals to justify treatment adjustments. Benefits and limitations of the tested in situ probes for the application of alert levels are discussed, and key knowledge gaps for future research and guidance are identified.
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Recently, in situ YSI EXO2 phycocyanin fluorescence probes have been widely deployed as a means to determine cyanobacterial abundance in drinking water sources, yet few studies have evaluated the effects of natural organic matter (NOM) and the ambient water temperature on the probe readings. In this study, Suwannee River NOM was added to laboratory cultivated cyanobacterial species to test the performance of the phycocyanin probe. The impact of temperature on phycocyanin fluorescence was evaluated by monitoring the laboratory cultivated cyanobacterial species and extracted phycocyanin pigment. Additionally, in situ phycocyanin fluorescence of the field samples from the water intake of a drinking water treatment plant (DWTP) in 2018 were compared with grab sample laboratory taxonomic analyses. We found: (1) the presence of Suwannee River NOM leads to the decrease in cell-bound cyanobacterial phycocyanin readings; (2) increasing ambient water temperature reduces dissolved and cell-bound cyanobacterial phycocyanin readings; (3) field study phycocyanin probe readings significantly correlated with the total cyanobacterial biovolume (R = 0.73, p < 0.1), and the relationship depends on the biovolume of dominant cyanobacterial species; (4) phycocyanin probe readings have a strong positive correlation with the natural light intensities; and (5) probe users should be fully aware of the sources of interferences when interpreting the results and apply the other physical-chemical parameters data simultaneously generated by the fluorometry to improve the probe’s measurements.