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The study was to investigate the change patterns of soil organic carbon (SOC), total nitrogen (TN), and soil C/N (C/N) in each soil sublayer along vegetation restoration in subtropical China. We collected soil samples in four typical plant communities along a restoration chronosequence. The soil physicochemical properties, fine root, and litter biomass were measured. Our results showed the proportion of SOC stocks (Cs) and TN stocks (Ns) in 20–30 and 30–40 cm soil layers increased, whereas that in 0–10 and 10–20 cm soil layers decreased. Different but well-constrained C/N was found among four restoration stages in each soil sublayer. The effect of soil factors was greater on the deep soil than the surface soil, while the effect of vegetation factors was just the opposite. Our study indicated that vegetation restoration promoted the uniform distribution of SOC and TN on the soil profile. The C/N was relatively stable along vegetation restoration in each soil layer. The accumulation of SOC and TN in the surface soil layer was controlled more by vegetation factors, while that in the lower layer was controlled by both vegetation factors and soil factors.